The Nature of Prophage in Lysogenic Bacillus Megatherium

نویسندگان

  • Elizabeth M. Miller
  • Walther F. Goebel
چکیده

1. By complement-fixation tests it has been shown that the lysogenic and sensitive strains of B. megatherium share one or more antigenic components which are serologically related but not identical. 2. Bacilli of the lysogenic strain of B. megatherium, when grown under conditions such that little extracellular phage is produced, fail to evoke antibodies in rabbits which react either in complement-fixation or neutralization tests with purified megatherium T phage. From this it must be concluded either that prophage is not antigenic or that any antibody which it might elicit does not react with the antigens of the mature virus. The observations reported in this communication accord with the hypothesis that prophage is a genetic structure.

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Observations on phage output and on anaerobic lysis of lysogenic strains of Bacillus megatherium.

The phenomenon of lysogenicity is an interesting aspect of the bacteriumbacteriophage relationship, for this is an instance of host cells capable of carrying virus through an infinite succession of subcultures, even through cycles of sporulation (den Dooren de Jong, 1930, 1932). Unlike other bacterium-bacteriophage systems, infection of the host does not inevitably terminate in its destruction;...

متن کامل

Factors Controlling the Production of Lysogenic Cultures of B. megatherium

(1) The proportion of infected B. megatherium cells which develop lysogenic colonies depends on the number and kind of infecting virus particles and on the culture medium in which the cells are growing. (2) Cells infected with 100 or more T virus particles (from megatherium 899) in yeast extract peptone disintegrate, produce very few virus particles, and less than one lysogenic colony per 10(7)...

متن کامل

Mutants of a Bacteriophage of Bacillus Megatherium

Repeated transfers in 5 per cent peptone or asparagine media of sensitive B. megatherium cultures containing C phage have made possible the isolation of lysogenic bacteria which produce different strains of phage. This is probably due to the failure of C virus to make the lysogenic adjustment while those of its variants which can succeed are selected. Four distinct plaque types have been isolat...

متن کامل

Mechanism of transfection with deoxyribonucleic acid from the temperate Bacillus bacteriophage phi-105.

Bacteriophage phi105 is a temperate phage for the transformable Bacillus subtilis 168. The infectivity of deoxyribonucleic acid (DNA) extracted from mature phi105 phage particles, from bacteria lysogenic for phi105 (prophage DNA), and from induced lysogenic bacteria (vegetative DNA) was examined in the B. subtilis transformation system. About one infectious center was formed per 10(8) mature DN...

متن کامل

Induction of prophage SPO2 in Bacillus subtilis: prophage excision in the absence of bacterial or bacteriophage DNA synthesis.

Bacillus subtilis lysogenic for SPO2 wild type was induced under conditions preventing synthesis of both bacterial and phage DNA. The infectivity of phage DNA in transfection is strongly decreased under these conditions, whereas the activity of single phage genes as measured by marker rescue with superinfecting phage is unaffected. DNA from induced cells was sedimented in neutral sucrose gradie...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

عنوان ژورنال:
  • The Journal of Experimental Medicine

دوره 100  شماره 

صفحات  -

تاریخ انتشار 1954